This Week in Ag #8

By Fred Nichols
Chief Marketing Officer,
Bio Huma Netics, Inc.

Farmers love to grow corn. Only twice in modern history has corn not held the title of the most-grown crop in the USA. That was in 2018 and (if you count it) 1983, when the government’s PIK program artificially swayed planting practices. Spurred by record fertilizer prices, many projected soybeans would overtake corn last year in their annual acreage tug-of-war. But the allure of maize once again prevailed. [Read more…]

This Week in Ag #6

By Fred Nichols
Chief Marketing Officer,
Bio Huma Netics, Inc.

Tomorrow is National Ag Day! Just like Bio Huma Netics, Inc., National Ag Day is enjoying its golden anniversary. The day is dedicated to celebrating and raising awareness for how our food, fiber (and energy) is produced. This year’s theme is “Growing a Climate for Tomorrow.” Agriculture is the USA’s largest employer, responsible for 23 million jobs – with about 2 million being actual farmers. While the number of farmers continues to decline, the job market in agriculture is booming. USDA expects to see almost 58,000 job openings per year for graduates with a bachelor’s degree or higher. The strongest job market is expected for plant scientists, food scientists, sustainable biomaterials specialists, water resources scientists and engineers, precision agriculture specialists, and farm-animal veterinarians. While Ag Day is officially celebrated on March 21, agriculture is the one industry we celebrate every day, three times a day. [Read more…]

Introducing New Humic Conversations Video Series

We are excited to introduce our new educational project, the Humic Conversations Video Series. As the name suggests, each episode of the series will focus on humic substances. Two leading researchers from our Humic Research Lab. Dr. Rich Lamar and Dr. Hiarhi Monda will share their expertise with the viewers.

In Episode 1 of this series, we discuss the basic science of humic substances. Dr. Lamar and Dr. Monda explain what humic and fulvic acids are, how they are formed, where they come from, and what their biostimulant properties are when it comes to plant and microbial growth.

The 16-minuite video is closed captioned in English and Spanish.

Research Report: Biostimulant Effect of Humic Acids on Tomato Plants Under Nutritional Stress

In a research study, originally published in Frontiers in Plant Science, May 2021, Vol. 12:660224, the biostimulant properties of humic acids (HA) were tested on Micro Tom tomato plants under increasing nutritional stress. The results confirmed the positive role humic acids play in enhancing nutrient efficiency uptake in plants.

A team of scientists from the Bio Huma Netics, Inc. (Hiarhi Monda, Ryan Fountain, and Rich Lamar) collaborated with Amy M. McKenna of the National High Magnetic Field Laboratory, Ion Cyclotron Resonance Facility, to conduct this research. The researchers believe that the increasing demands for biostimulants in the agricultural market requires the industry to provide research-based data on the efficiency and safety of this particular product category. This was the motivation that led them to initiate this extensive research. [Read more…]

Why Are Humic Substances Called Acids?

By Richard Lamar, PhD
Senior Director of Humic Research
Bio Huma Netics, Inc.

We are accustomed to seeing humic substances (humic and fulvic) in dry/granular form, and we tend to think of acids as liquids. So why are humic and fulvic substances called acids?All substances, solid AND liquid, have a chemical makeup. An acid is a chemical that can donate a proton (H+) to a water molecule (H2O, which would form H3O+) or to another chemical such as ammonia (NH3, which would form NH4+).

[Read more…]

What Differentiates Humic and Fulvic Acids?

By Richard Lamar, PhD
Director of Humic Research
Bio Huma Netics, Inc.

For centuries, humic acids (HA) were thought to be composed of much larger molecules than those found in fulvic acids (FA). However, the application of Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS), which separates molecules on the basis of molecular weight, demonstrates that the molecular weights of the two fractions both fall in the range of 200–800 Daltons (Da), with most of the molecules having molecular weights in the range of 200–400 Da (Figure 1). To give context, carbon (C) weighs 12 Da, oxygen (O) weighs 16 Da, and hydrogen (H) weighs 1 Da. Thus, phenol molecules (an aromatic organic compound, also called carbolic acid), which have 6 C, 1 O, and 11 H atoms, weigh 99 Da. [Read more…]

JoVE Video Journal Publication: Quantification of Humic and Fulvic Acids

Dr. Richard T. Lamar and Dr. Hiarhi Monda of our Humic Research Laboratory, with assistance from analytical chemist Ryan Fountain, have published a methodology video in the biochemistry section of the peer-reviewed online video journal, JoVE.

The video, Quantification of Humic and Fulvic Acids in Humate Ores, DOC, Humified Materials and Humic Substance-Containing Commercial Products, shows the step-by-step laboratory methodology (the New Standard Method) for gravimetric quantification of humic substances (e.g., humic and fulvic acids) on an ash-free basis, in dry and liquid materials from soft coals (i.e., oxidized and non-oxidized lignite and sub-bituminous coal), humate ores and shales, peats, composts and commercial fertilizers and soil amendments.

In the video introduction, Dr. Lamar states, “The New Standard Method for quantification of humic acids provides a more accurate and precise analysis compared to the existing regulatorily accepted methods, and it also provides a standard method for pure hydrophobic fulvic acid quantification. The advantage of this protocol is that it provides a gravimetric analysis of humic and hydrophobic fulvic acid concentrations on an ash-free basis, and the extraction process has been optimized to obtain the highest recoveries of both humic and fulvic acids from samples.

At the video’s conclusion, Dr. Monda states, “Following this procedure, the dry humic and fulvic acids obtained can be used for characterization purposes, such as the carbon-13 and the proton NMR electron resonance, and the ultrahigh resolution mass spectrometry, among other useful techniques. This can be used for characterization of the humus chemistry, as well as being a useful tool to dig deep into the structure-activity relationship with plant fitness and the underlying plant defense mechanisms.

Direct link to video on the JoVE Website: https://www.jove.com/v/61233/quantification-humic-fulvic-acids-humate-ores-doc-humified-materials (A free subscription will be required to view the entire video on the JoVE Website.)

From the JoVE Website: Filmed at the world’s top scientific institutions, JoVE videos bring to life the intricate details of cutting-edge experiments enabling efficient learning and replication of new research methods and technologies. JoVE is a peer-reviewed scientific video journal that is indexed in PubMed and Web of Science.

Video: Mixing Liquid Humic Acid with Agrochemicals

This video demonstrates how to mix liquid humic acids, such as Huma Pro® 16, with liquid fertilizers without creating precipitation that can gum up spray or irrigation equipment. [Read more…]

Humic Acid Biostimulant Research by BHN Humic R&D Lab Scientists Published in Int’l Agronomic Journal

In its May 2021 issue, Frontiers in Plant Science published a research article by BHN Humic R&D Lab scientists Dr. Hiarhi Monda, Ryan Fountain, and Dr. Richard T. Lamar in collaboration with Dr. Amy McKenna of the National High Magnetic Field Laboratory, Ion Cyclotron Resonance Facility, Tallahassee, Fla.

The research, titled “Bioactivity of Humic Acids Extracted from Shale Ore: Molecular Characterization and Structure-Activity Relationship With Tomato Plant Yield Under Nutritional Stress,” revealed an unprecedented level of molecular characterization made possible by means of ultra-high resolution ion cyclotron mass spectrometry, providing a more comprehensive understanding of individual components of humic substances involved in enhancing plant productivity.

A detailed characterization of chemical composition becomes critical as new government regulations of biostimulant products will require the elucidation of the mode of action in order to supply farmers with effective product claims based on science.

The objective of this study was to investigate in detail the chemical features of humic acids (HAs) extracted from sedimentary ore with the aim of exploring the potential relationship of chemical functions with biostimulant activity and to evaluate the extent to which the priming effect of HAs on tomato plants under nutritional stress was reflected on the yield gains. 

The results of this study proved the biostimulant efficacy of humic acid application that improved nutrient use efficiency and at the same time alleviated the nutritional stress condition. All tomato plants treated with humic acids showed faster adaptation to stress conditions, particularly when nutrient deficiency occurred. Plant growth and tomato yield increased when provided with humic acids under low nutritional doses, and tomato fruit quality was improved under all humic acid treatments.

The increased antioxidants production under humic application has been correlated to the presence of specific molecules in the humic extract. These molecules, such as quinones and flavonoids, can act as both antioxidants and pro-oxidants that can trigger the plant defensive system, ultimately leading to a fast and effective response to nutrient deficiency with a consequent enhancement of plant morphology and productivity.

Among the study’s conclusions are that plant pre-conditioning with humic substances might represent an important determinant in the adaptive plant defense response and an effective strategy to improve nutrients management and plant yield.

The complete open-access article is available at https://www.frontiersin.org/articles/10.3389/fpls.2021.660224/full.

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